Induction of Potent Th1‐Type Immune Responses from a Novel DNA Vaccine for West Nile Virus New York Isolate (WNV‐NY1999)
2001; Oxford University Press; Volume: 184; Issue: 7 Linguagem: Inglês
10.1086/323395
ISSN1537-6613
AutoresJoo‐Sung Yang, J. Joseph Kim, Daniel S. Hwang, Andrew Y. Choo, Kesen Dang, Henry Maguire, Sagar B. Kudchodkar, Mathura P. Ramanathan, David B. Weiner,
Tópico(s)Virology and Viral Diseases
ResumoWest Nile virus (WNV) is a vectorborne pathogen that induces brain inflammation and death. Recently, confirmed cases of infection and deaths have occurred in the United States Mid-Atlantic region. In this study, a DNA vaccine encoding the WNV capsid protein was constructed, and the in vivo immune responses generated were investigated in DNA vaccine–immunized mice. Antigen-specific humoral and cellular immune responses were observed, including a potent induction of antigen-specific Th1 and cytotoxic T lymphocyte responses. Strong induction of Th1-type immune responses included high levels of antigen-specific elaboration of the Th1-type cytokines interferon-γ and interleukin-2 and β-chemokines RANTES (regulated upon activation, normal T cell-expressed and secreted) and macrophage inflammatory protein–1β. Dramatic infiltration of CD4 and CD8 T cells and macrophages also was observed at the muscle injection site. These results support the potential utility of this method as a tool for developing immunization strategies for WNV and other emerging pathogens
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