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Isolation and DNA-binding characteristics of a protein involved in transcription activation of two divergently transcribed, essential yeast genes.

1989; Springer Nature; Volume: 8; Issue: 10 Linguagem: Inglês

10.1002/j.1460-2075.1989.tb08453.x

1460-2075

Hartmut Halfter, Ulrike Müller, E.‐L. Winnacker, Dieter Gallwitz,

Genomics and Chromatin Dynamics

Research Article1 October 1989free access Isolation and DNA-binding characteristics of a protein involved in transcription activation of two divergently transcribed, essential yeast genes. H. Halfter H. Halfter Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author U. Müller U. Müller Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author E.L. Winnacker E.L. Winnacker Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author D. Gallwitz D. Gallwitz Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author H. Halfter H. Halfter Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author U. Müller U. Müller Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author E.L. Winnacker E.L. Winnacker Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author D. Gallwitz D. Gallwitz Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. Search for more papers by this author Author Information H. Halfter1, U. Müller1, E.L. Winnacker1 and D. Gallwitz1 1Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Genetics, Göttingen, FRG. The EMBO Journal (1989)8:3029-3037https://doi.org/10.1002/j.1460-2075.1989.tb08453.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have identified a protein, BAF1, which has two oppositely oriented, partially overlapping binding sites within a symmetrical sequence located midway between and upstream of the divergently transcribed YPT1 and TUB2 genes of the yeast Saccharomyces cerevisiae. The 120 kd BAF1 protein was purified to near homogeneity and used to delineate the two binding sites and to identify apparent protein contact sites by the missing contact technique, methylation interference and by site-directed mutagenesis. The BAF1-recognition sequence contains a conserved TCN7ACG element recently identified at autonomously replicating sequences (ARS) and in the 5′ and 3′ flanking region of other yeast genes. The symmetrical sequence of the YPT1/TUB2 intergene region seems not to be involved in DNA replication but activates transcription in an orientation-independent fashion. Previous ArticleNext Article Volume 8Issue 101 October 1989In this issue RelatedDetailsLoading ...