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Optimisation of Y-STR multiplexing combining established and newly described loci

2003; Elsevier BV; Volume: 1239; Linguagem: Inglês

10.1016/s0531-5131(02)00225-x

1873-6157

Sandra Beleza, Anna González‐Neira, Ángel Carracedo, António Amorim, Leonor Gusmão,

Genetic diversity and population structure

In order to extend Y databases for forensic and anthropological applications, some recently described markers were selected to be co-amplified with established ones in a multiplex reaction. The markers chosen were DYS391/434/437/439, with non-overlapping size ranges for the alleles of each marker, allowing its use in both monochromatic and polychromatic platforms. For the design of this tetraplex, new primers were used and several technical conditions were assayed. This newly constructed tetraplex has proved to be very useful in population genetic studies and forensic casework.