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Production of amylases by Aspergillus tamarii

1999; Sociedade Brasileira de Microbiologia; Volume: 30; Issue: 2 Linguagem: Inglês

10.1590/s0001-37141999000200014

1678-9881

Fabiana Guillen Moreira, Fernanda Aparecida de Lima, Sophia Renata Fazzano Pedrinho, Veridiana Lenartovicz, Cristina Giatti Marques de Souza, Rosane Marina Peralta,

Biofuel production and bioconversion

A strain of Aspergillus tamarii, a filamentous fungus isolated from soil, was able to produce both <FONT FACE="Symbol">a</FONT>-amylase and glucoamylase activities in mineral media supplemented with 1% (w/v) starch or maltose as the carbon source. Static cultivation led to significantly higher yields than those obtained using shaking culture. The production of amylases was tolerant to a wide range of initial culture pH values (from 4 to 10) and temperature (from 25 to 42oC). Two amylases, one <FONT FACE="Symbol">a</FONT>-amylase and one glucoamylase, were separated by ion exchange chromatography. Both partially purified enzymes had optimal activities at pH values between 4.5 and 6.0 and were stable under acid conditions (pH 4.0-7.0). The enzymes exhibited optimal activities at temperatures between 50o and 60o C and were stable for more than ten hours at 55oC.