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Induction of cellular immune response and anti-Salmonella enterica serovar typhi bactericidal antibodies in healthy volunteers by immunization with a vaccine candidate against typhoid fever

2004; Elsevier BV; Volume: 93; Issue: 2-3 Linguagem: Inglês

10.1016/j.imlet.2004.01.010

1879-0542

Rosa Marı́a Salazar-González, Carmen Maldonado‐Bernal, Nora Elena Ramı́rez-Cruz, Nora Ríos‐Sarabia, Jorge Beltrán-Nava, Jorge Alberto Castañón-González, Noemí Patricia Castillo-Torres, José Antonio Palma‐Aguirre, Manuel Carrera-Camargo, Constantino López‐Macías, Armando Isibasi,

Bacterial Infections and Vaccines

Typhoid fever remains a serious public health problem. We have developed a vaccine from Salmonella enterica serovar typhi (S. typhi) outer-membrane proteins (OMPs) known as porins. A single subcutaneous dose of 10 μg of porins induced a five-fold (P=0.05) seroconversion index consisting of IgM and IgG at 7 and 15 days after vaccination as well as the production of IgG1 and IgG2 isotypes. The porins-based vaccine induced a two-fold increase (P=0.05) in bactericidal titres in volunteers, whom also developed a T-cell response characterized by the production of interferon-γ (INF-γ). Side effects after vaccination were mild and transient. The data showed that our S. typhi porins-based candidate vaccine is safe and immunogenic in healthy humans.