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Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni

2003; Nature Portfolio; Volume: 35; Issue: 2 Linguagem: Inglês

10.1038/ng1237

1546-1718

Sergio Verjovski‐Almeida, Ricardo DeMarco, Elizabeth A. L. Martins, Pedro Edson Moreira Guimarães, Elida P.B. Ojopi, Apuã C.M. Paquola, João Paulo Piazza, Milton Yutaka Nishiyama, João Paulo Kitajima, Rachel Adamson, Peter D. Ashton, Maria F. Bonaldo, Patricia S. Coulson, G. Dillon, Leonardo Paiva Farias, Sheila P. Gregório, Paulo Lee Ho, Ricardo A. Leite, Luiz Cosme Cotta Malaquias, Regina Célia Pereira Marques, Patrícia A. Miyasato, Ana L. T. O. Nascimento, Fernanda Pires Ohlweiler, Eduardo M. Reis, Marcela Alves Ribeiro, Renata Guerra de Sá, Gaëlle C Stukart, Marcelo B. Soares, Cybele Gargioni, Toshie Kawano, Vanderlei Rodrigues, Alda Maria Backx Noronha Madeira, R. Alan Wilson, Carlos Frederico Martins Menck, João Carlos Setúbal, Luciana C. C. Leite, Emmanuel Dias‐Neto,

Genetics, Aging, and Longevity in Model Organisms

Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.