Solid‐State Protein‐Structure Determination with Proton‐Detected Triple‐Resonance 3D Magic‐Angle‐Spinning NMR Spectroscopy
2007; Wiley; Volume: 46; Issue: 44 Linguagem: Inglês
10.1002/anie.200702905
ISSN1521-3773
AutoresDonghua H. Zhou, John J. Shea, Andrew J. Nieuwkoop, W. Trent Franks, Benjamin J. Wylie, Charles Mullen, Dennis Sandoz, Chad M. Rienstra,
Tópico(s)Advanced MRI Techniques and Applications
ResumoAngewandte Chemie International EditionVolume 46, Issue 44 p. 8380-8383 Communication Solid-State Protein-Structure Determination with Proton-Detected Triple-Resonance 3D Magic-Angle-Spinning NMR Spectroscopy† Donghua H. Zhou Dr., Donghua H. Zhou Dr. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorJohn J. Shea, John J. Shea Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorAndrew J. Nieuwkoop, Andrew J. Nieuwkoop Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorW. Trent Franks, W. Trent Franks Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorBenjamin J. Wylie, Benjamin J. Wylie Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorCharles Mullen, Charles Mullen Varian, Inc. Fort Collins, CO, USASearch for more papers by this authorDennis Sandoz, Dennis Sandoz Varian, Inc. Palo Alto, CA, USASearch for more papers by this authorChad M. Rienstra Prof. Dr., Chad M. Rienstra Prof. Dr. [email protected] Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this author Donghua H. Zhou Dr., Donghua H. Zhou Dr. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorJohn J. Shea, John J. Shea Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorAndrew J. Nieuwkoop, Andrew J. Nieuwkoop Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorW. Trent Franks, W. Trent Franks Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorBenjamin J. Wylie, Benjamin J. Wylie Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this authorCharles Mullen, Charles Mullen Varian, Inc. Fort Collins, CO, USASearch for more papers by this authorDennis Sandoz, Dennis Sandoz Varian, Inc. Palo Alto, CA, USASearch for more papers by this authorChad M. Rienstra Prof. Dr., Chad M. Rienstra Prof. Dr. [email protected] Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Fax: (+1) 217-244-4655 http://www.scs.uiuc.edu/∼rienstraSearch for more papers by this author First published: 02 November 2007 https://doi.org/10.1002/anie.200702905Citations: 195 † This research was supported by the National Institutes of Health (R01 GM-75937 to C.M.R). We thank Philippe Nadaud and Prof. Christopher Jaroniec (Ohio State University) for advice regarding expression of deuterated GB1, and Mircea Cormos and John A. Stringer (Varian, Inc.) for advice on probe performance. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state 1H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. Citing Literature Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z702905_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume46, Issue44November 12, 2007Pages 8380-8383 RelatedInformation
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