The structure of nucleosome core particles as revealed by difference Raman spectroscopy
1986; Oxford University Press; Volume: 14; Issue: 6 Linguagem: Inglês
10.1093/nar/14.6.2583
ISSN1362-4962
AutoresHideyuki Hayashi, Yoshifumi Nishimura, Masato Katahira, Masamichi Tsuboi,
Tópico(s)RNA and protein synthesis mechanisms
ResumoRaman spectra have been observed of nucleosome core particles (I) prepared from chicken erythrocyte chromatln, Its isolated 146 bp DNA (II), and its Isolated histone octamer (H2A+H2B+H3+H4) 2 (III). By examining the difference Raman spectra, (I) − (II), (I)−(III), and (I)−(II)−(III), several pieces of information have been obtained on the conformation of the DNA moiety, the conformation of the histone moiety, and the DNA-histone Interaction in the nucleosome core particles. In the nucleosome core particles, about 15 bp (A.T rich) portions of the whole 146 bp DNA are considered to take an A-form conformation. These are considered to correspond to its bent portions which appear at intervals of 10 bp.
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