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Donor-specific antibody detection: comparison of single antigen assay and Luminex crossmatches

2010; Wiley; Volume: 76; Issue: 5 Linguagem: Inglês

10.1111/j.1399-0039.2010.01531.x

1399-0039

José Luis Caro-Oleas, María Francisca González‐Escribano, S. Toro-Llamas, Maria Acevedo-Calado, Maria José Martinez‐Bravo, Isabel Aguilera, Antonio Núñez‐Roldán,

Complement system in diseases

Luminex bead-based assays are routinely used in the study of anti-human leukocyte antigen (HLA) donor-specific antibodies (DSA). Single antigen (SA) assays use beads coated with recombinant antigens whereas Luminex crossmatch (Xm-DSA) tests consist of beads coated with isolated donor-specific HLA molecules. The aim of this study was to compare these techniques used to detect DSA. A total of 24 sera recognizing different HLA class I (seven anti-HLA-A and seven anti-HLA-B) as well as class II (seven anti-HLA-DR and three anti-HLA DQ) specificities by complement dependent cytotoxicity were included in the study. These sera were used undiluted and in serial dilutions to perform both class I and II SA and Xm-DSA assays. In the case of Xm-DSA the same serum was checked with different lysates. A total of 42 lysates were used to perform a total of 61 crossmatches: 42 to detect anti-class I and 19 to detect anti-class II antibodies. The maximum positive dilution was higher for SA in 76% of the class I and in 90% of the class II crossmatches. Those cases with a higher sensitivity of the Xm-DSA could not be explained by a larger number of antigen targets.