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Expression in vivo of CD45RA, CD45RB and CD44 on T cell receptor‐transgenic CD8 + T cells following immunization

1995; Wiley; Volume: 25; Issue: 6 Linguagem: Inglês

10.1002/eji.1830250640

1521-4141

Maria Pihlgren, Liz Lightstone, Clio Mamalaki, Galia Rimon, Dimitris Kioussis, Jacqueline Marvel,

Immune Cell Function and Interaction

Abstract We used mice transgenic for a major histocompatibility complex class I‐restricted T cell receptor to study the changes of phenotype in vivo which follow priming by antigen of CD8 T cells. We show that following priming with peptide, CD44 on CD8 T cells is up‐regulated. The change of phenotype was relatively stable, as primed CD8 cells isolated from thymectomized mice 6 weeks after priming still expressed increased levels of CD44. CD8 T cells in these mice are still responsive to peptide and could represent long‐lived primed cells. No downregulation in vivo of the CD45RA or CD45RB isoforms was found, indicating that there is a differential regulation of the expression of CD44 and CD45RB by activated CD8 transgenic T cells. These results contradict earlier studies in vitro which showed that CD8 T cells which have been primed earlier belong to the CD45RA − or CD45RB − subset.