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Identification of a Promoter Region Generating Sry Circular Transcripts Both in Germ Cells from Male Adult Mice and in Male Mouse Embryonal Gonads1

1997; Oxford University Press; Volume: 57; Issue: 5 Linguagem: Inglês

10.1095/biolreprod57.5.1128

1529-7268

Susanna Dolci, Paola Grimaldi, Raffaele Geremia, Maurizio Pesce, Pellegrino Rossi,

Animal Genetics and Reproduction

The mouse testis determining gene Sry is expressed in somatic cells of the differentiating male gonad as a linear transcript, encoding a transcription factor containing an HMG box. In the adult mouse testis, Sry expression occurs in meiotic and postmeiotic germ cells. The mouse genomic Sry locus is characterized by two arms of a large inverted repeat, flanking a unique region that, between an acceptor and a donor splice site, contains a single exon encoding the Sry protein. In germ cells from the adult mouse testis, Sry RNA is a circular molecule, which is generated by an inverted splicing event that utilizes the above-mentioned splice sites. Thus, a circular exon is spliced out starting from a large linear RNA precursor containing both arms of the inverted repeat, which pair and generate a large stemloop structure.