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A Universal Method for the Preparation of Covalent Protein–DNA Conjugates for Use in Creating Protein Nanostructures

2007; Wiley; Volume: 46; Issue: 46 Linguagem: Inglês

10.1002/anie.200701942

1521-3773

Benjamin P. Duckworth, Yan Chen, James W. Wollack, Yuk Y. Sham, Joachim D. Mueller, T. Andrew Taton, Mark D. Distefano,

Monoclonal and Polyclonal Antibodies Research

Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein–DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein–DNA hybrid structures with useful structural and functional features.