Synthesis of the extracellular Ig domain I of Emmprin carrying a chitobiose unit
2001; Elsevier BV; Volume: 42; Issue: 16 Linguagem: Inglês
10.1016/s0040-4039(01)00342-2
ISSN1873-3581
AutoresHironobu Hojo, Jun Watabe, Yoshiaki Nakahara, Yuko Nakahara, Yukishige Ito, Kazuki Nabeshima, Bryan P. Toole,
Tópico(s)Antimicrobial Peptides and Activities
ResumoThe extracellular Ig domain I of Emmprin (34-94) carrying a chitobiose unit at Asn44 was chemically synthesized. Boc-Asn with a benzyl-protected chitobiose unit was synthesized and used for the preparation of peptide thioester with the sequence of Emmprin (34-58) by Boc strategy. C-Terminal peptide amide (59-94) was also prepared by the solid-phase method. These segments were condensed by activation of the thioester group by silver ions to obtain a protected form of Emmprin (34-94)-NH2. After deprotection and air oxidation, the desired Emmprin (34-94)-NH2 with chitobiose was successfully obtained.
Referência(s)