Portal de Periódicos da CAPES

Localization of Single Avidin–Biotin Interactions Using Simultaneous Topography and Molecular Recognition Imaging

2005; Wiley; Volume: 6; Issue: 5 Linguagem: Inglês

10.1002/cphc.200400545

1439-7641

Andreas Ebner, Ferry Kienberger, Gerald Kada, Cordula M. Stroh, M. Geretschlàger, A. S. M. Kamruzzahan, Linda Wildling, William T. G. Johnson, Brian Ashcroft, Jeremy J. Nelson, Stuart Lindsay, Hermann J. Gruber, Peter Hinterdorfer,

Mechanical and Optical Resonators

Two-for-one: The authors discuss a powerful new tool that, in a single dynamic force microscopy scan, maps the topography of a surface and simultaneously recognizes specific target sites (see figure). Topography and recognition images correlated nicely at a lateral resolution of a few nanometers. This clever technique has great potential for a broad range of applications involving the detection of particular binding sites in native biological systems.