RFLP analysis of PCR-amplified small subunit ribosomal DNA of three fish microsporidian species
2000; Cambridge University Press; Volume: 120; Issue: 2 Linguagem: Inglês
10.1017/s0031182099005405
ISSN1469-8161
AutoresJ. Leiro, María-Isabel González-Siso, Anabel Paramá Díaz, Florencio M. Ubeira, M. L. Sanmartín,
Tópico(s)Myxozoan Parasites in Aquatic Species
ResumoThe phylogenetic relationships of the microsporidian species Microgemma caulleryi , Pleistophora finisterrensis and Tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (RFLP) analysis of PCR-amplified small-subunit rDNA (SSUrDNA). Using PCR primers specific for microsporidian SSUrDNA, a single product was obtained from each species, and heteroduplex analysis indicated a high degree of sequence homology among the 3 products. In RFLP analysis of the PCR-amplified SSUrDNA, the enzymes Alu I and Dde I gave restriction patterns that differed among all 3 species. Phylogenetic analysis using restriction patterns as differential characters indicated that Microgemma caulleryi and Tetramicra brevifilum are more closely related to each other than to Pleistophora finisterrensis .
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