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The Lysis of Cholera and El Tor Vibrios

1969; Microbiology Society; Volume: 59; Issue: 1 Linguagem: Inglês

10.1099/00221287-59-1-91

2059-9323

P. C. Adhikari, C. Raychaudhuri, Sohini Chatterjee,

Metabolomics and Mass Spectrometry Studies

SUMMARY: Vibrio cholerae and V. eltor underwent considerable lysis after treatment with tris+EDTA, tris+EDTA+lysozyme or sodium lauryl sulphate. Lysis induced by tris+EDTA or tris+EDTA+lysozyme was completely inhibited by 0·3 m-sucrose, 0·15 m-NaCl or 0·01 m-MgCl2. The extent of death after treatment with tris+EDTA depended on EDTA concentration: 10 min. with 10 μg. EDTA/ml. left 43 to 50% of V. eltor viable and 71 to 75% of V. cholerae. Labelled organisms exhibited maximum leakage of 32P compounds when exposed to 71% (v/v) ethanol for V. cholerae and 50% (v/v) ethanol for V. eltor; leakage in 100% ethanol was about 77% of maximum for V. cholerae and 66% for V. eltor. Both 32P labelled and unlabelled vibrios released considerable amounts of nucleic acid, phospholipid, protein and carbohydrate and practically all their acid soluble phosphates with EDTA.