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Superoxide and Nitric Oxide Production of Kuruma Prawn ( Marsupenaeus japonicus ) Hemocytes

2002; Springer Science+Business Media; Volume: 68; Issue: sup2 Linguagem: Inglês

10.2331/fishsci.68.sup2_1119

1444-2906

Toshiaki Itami, Yasuhiro Ohno, Nobutaka Suzuki, Kenichi Doi, Masakazu Kondo, Yukinori Takahashi, Minoru Maëda, Gen‐Ichiro Soma, Hiroyuki Inagawa, Teruko Honda, Yuichi Yokomizo,

Neurobiology and Insect Physiology Research

The Cypridina luciferin analogue (CLA), a highly sensitive chemiluminescence (CL) probe, was used to detect superoxide (O2) in kuruma prawn hemocytes. The CLA-dependent CL of the shrimp hemocyte after phorbol 12-myristate 13-acetate (PMA) stimulation was inhibited by superoxide dismutase. Nitric oxide (NO) production from the shrimp hemocytes was detected by fluorometric measurement after co-stimulation with lipopolysaccharides (LPS) and recombinant human interferon γ in vitro. The supernatant of a hemocyte culture after stimulation showed an increase in the fluorescence intensity, and this intensity decreased with the addition of NG-monomethyl-L-arginine (L-NMMA). These results show that shrimp hemocytes can produce O2 and NO and these free radicals play an important role in the shrimp defense network.