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Chaga mushroom (Inonotus obliquus ) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells

2008; Baishideng Publishing Group; Volume: 14; Issue: 4 Linguagem: Inglês

10.3748/wjg.14.511

2219-2840

Myung‐Ja Youn, Jin‐Kyung Kim, Seong-Yeol Park, Yunha Kim, Se-Jin Kim, Jin Seok Lee, Kyu Yun Chai, Hye‐Jung Kim, Ming‐Xun Cui, Hong Seob So, Ki‐Young Kim, Raekil Park,

Natural product bioactivities and synthesis

AIM:To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus ) water extract on human hepatoma cell lines, HepG2 and Hep3B cells. METHODS: The cytotoxicity of Chaga extract was s c r e e n e d b y 3 -[ 4 , 5 -d i m e t h y l t h i a z o l -2 -y l ] -2 , 5 -d i p h e n y l t e t r a z o l i u m b r o m i d e ( M TT ) a s s a y.Morphological observation, flow cytometry analysis, Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract.RESULTS: HepG2 cells were more sensitive to Chaga extract than Hep3B cells, as demonstrated by markedly reduced cell viability.Chaga extract inhibited the cell growth in a dose-dependent manner, which was accompanied with G0/G1-phase arrest and apoptotic cell death.In addition, G0/G1 arrest in the cell cycle was closely associated with down-regulation of p53, pRb, p27, cyclins D1, D2, E, cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 expression. CONCLUSION:Chaga mushroom may provide a new therapeutic option, as a potential anticancer agent, in the treatment of hepatoma.