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Using Mass Spectrometry to Characterize Self‐Assembled Monolayers Presenting Peptides, Proteins, and Carbohydrates

2002; Wiley; Volume: 41; Issue: 24 Linguagem: Inglês

10.1002/anie.200290026

1521-3773

Jing Su, Milan Mrksich,

Advanced Proteomics Techniques and Applications

Peptide and carbohydrate biochips can be assayed with MALDI mass spectrometry. Self-assembled monolayers (SAMs) that are modified with ligands provide a platform for identifying selective protein–ligand and enzyme–substrate interactions. MALDI provides a rapid and sensitive method for detection and avoids the need to label protein analytes with fluorescent or radioactive tags. The picture shows the mass spectrum of a SAM presenting a carbohydrate.