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The SecA Subunit of Escherichia coli Preprotein Translocase Is Exposed to the Periplasm

1998; American Society for Microbiology; Volume: 180; Issue: 21 Linguagem: Inglês

10.1128/jb.180.21.5776-5779.1998

1098-5530

Jerry Eichler, William Wickner,

Escherichia coli research studies

ABSTRACT SecA undergoes conformational changes during translocation, inserting domains into and across the membrane or enhancing the protease resistance of these domains. We now show that some SecA bound at SecYEG is accessible from the periplasm to a membrane-impermeant probe in cells with a permeabilized outer membrane but an intact plasma membrane.