Aptamer-based surface-enhanced Raman scattering detection of ricin in liquid foods
2011; Royal Society of Chemistry; Volume: 2; Issue: 8 Linguagem: Inglês
10.1039/c1sc00201e
ISSN2041-6539
AutoresLili He, Elise A. Lamont, Belamaranahally Veeregowda, Srinand Sreevatsan, Christy L. Haynes, Francisco Diez‐Gonzalez, Theodore P. Labuza,
Tópico(s)Biosensors and Analytical Detection
ResumoA "two-step" aptamer-based surface-enhanced Raman scattering (SERS) detection assay was developed for ricin in liquid foods. Ricin B chain was first captured out of food matrices by aptamer-conjugated silver dendrites and then the spectrum was directly read on the silver dendrites. Aptamer use in this assay promotes ease of manipulation as well as improved sensitivity compared to antibody-based approaches. The limit of detection for ricin B chain was 10 ng mL−1 in phosphate buffered saline (PBS), 50 ng mL−1 in orange juice, and 100 ng mL−1 in milk based on principal component analysis (PCA) of measured spectra. This assay shows great promise as a rapid (< 40 min), sensitive, and simple "Yes/No" method to detect bio-weapons like ricin in liquid foods.
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