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Dual staining of corneal endothelium with trypan blue and alizarin red S: importance of pH for the dye-lake reaction.

1981; BMJ; Volume: 65; Issue: 12 Linguagem: Inglês

10.1136/bjo.65.12.815

1468-2079

M.J. Taylor, Charles J. Hunt,

Retinal and Optic Conditions

Evaluation of corneal endothelial integrity by combined staining with the vital stain trypan blue and the intercellular stain alizarin red S provides a simple, quick technique for visualisation of both damaged and normal cells, thereby permitting the quantification of endothelial cell damage. Adjustment of the pH of the alizarin red S reagent to 4.2 is important for optimum dye-laking at the intercellular borders, and brief fixation with glutaraldehyde maintains the staining effect of both dyes.