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Digestive proteinases of Artemesia longinaris (Decapoda, Penaeidae) and relationship with molting

2002; Elsevier BV; Volume: 132; Issue: 3 Linguagem: Inglês

10.1016/s1096-4959(02)00080-5

1879-1107

Analía Verónica Fernández‐Gimenez, Fernando García‐Carreño, M. Ángeles Navarrete del Toro, Jorge L. Fenucci,

Protein Hydrolysis and Bioactive Peptides

Digestive proteinase activities of Artemesia longinaris were assayed at different stages of the molting cycle. Total proteolytic activity in the hepatopancreas was highest during postmolt. Trypsin and chymotrypsin activities were highest during intermolt. Specific inhibitors and zymograms of A. longinaris hepatopancreas extracts showed four trypsins (14.79, 15.49, 16.60, 17.38 kDa, respectively) and three chymotrypsins (21.38, 22.91, 27.54 kDa, respectively). Our results suggest that proteolytic activity in the hepatopancreas of A. longinaris is influenced by the molting cycle. Types and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.