MCF-10A-NeoST: a new cell system for studying cell-ECM and cell-cell interactions in breast cancer.
2001; National Institutes of Health; Volume: 7; Issue: 11 Linguagem: Inglês
Autores
Nicole D. Zantek, Jennifer Walker‐Daniels, Jane C. Stewart, Rhonda K. Hansen, Dan R. Robinson, Hui Miao, Binghe Wang, Hsing‐Jien Kung, Mina J. Bissell, Michael S. Kinch,
Tópico(s)Axon Guidance and Neuronal Signaling
ResumoThere is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers.We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer.NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells.MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.
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