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Analysis of solvent tolerance in Pseudomonas putida DOT‐T1E based on its genome sequence and a collection of mutants

2012; Wiley; Volume: 586; Issue: 18 Linguagem: Inglês

10.1016/j.febslet.2012.07.031

1873-3468

Zulema Udaondo, Estrella Duque, Matilde Fernández, Lázaro Molina, Jesús de la Torre, Patricia Bernal, José‐Luis Niqui, Cecilia Pini, Amalia Roca, Miguel A. Matilla, María Antonia Molina‐Henares, Hortencia Silva‐Jiménez, Gloria Navarro‐Avilés, Andreas Büsch, Jesús Lacal, Tino Krell, Ana Segura, Juan L. Ramos,

Genomics and Phylogenetic Studies

Pseudomonas putida strains are prevalent in a variety of pristine and polluted environments. The genome of the solvent-tolerant P. putida strain DOT-T1E which thrives in the presence of high concentrations of monoaromatic hydrocarbons, contains a circular 6.3 Mbp chromosome and a 133 kbp plasmid. Omics information has been used to identify the genes and proteins involved in solvent tolerance in this bacterium. This strain uses a multifactorial response that involves fine-tuning of lipid fluidity, activation of a general stress-response system, enhanced energy generation, and induction of specific efflux pumps that extrude solvents to the medium. Local and global transcriptional regulators participate in a complex network of metabolic functions, acting as the decision makers in the response to solvents.