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Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China

2009; Microbiology Society; Volume: 59; Issue: 6 Linguagem: Inglês

10.1099/ijs.0.004713-0

1466-5034

Jianping Guo, Yuexin Lin, Miao Zhao, Rui Sun, T. T. Wang, Ming Tang, Ge Hong Wei,

Plant Disease Resistance and Genetics

The taxonomic position of an actinomycete isolated from a lead-polluted soil in Gansu province, north-west China, was determined by using a polyphasic approach. Chemical and morphological properties of the isolate, designated strain CCNWHX 13-160T, were similar to those of streptomycetes. Analysis of the almost complete 16S rRNA gene sequence placed strain CCNWHX 13-160T in the genus Streptomyces where it formed a distinct phyletic line with recognized Streptomyces species. The strain was most similar to Streptomyces pseudovenezuelae NBRC 12904T (98.9 %) and Streptomyces resistomycificus NBRC 12814T (98.8 %). Furthermore, DNA–DNA hybridization studies between the novel isolate and these two strains showed relatedness values of 49.7±0.8 and 43.2±1.1 %, respectively. It is proposed that strain CCNWHX 13-160T (=ACCC 41207T=HAMBI 2991T) be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces plumbiresistens sp. nov. The MIC of Pb2+ for growth of strain CCNWHX 13-160T was 4.0 mM.