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Artigo Revisado por pares
BIBTEX RIS

Fluorescent Indicators for Imaging Nitric Oxide Production

1999; Wiley; Volume: 38; Issue: 21 Linguagem: Inglês

10.1002/(sici)1521-3773(19991102)38

ISSN

1521-3773

Autores

Hirotatsu Kojima, Yasuteru Urano, Kazuya Kikuchi, Tsunehiko Higuchi, Yasunobu Hirata, Tetsuo Nagano,

Tópico(s)

Eicosanoids and Hypertension Pharmacology

Resumo

The membrane-permeating indicator DAF-FM DA is transformed by intracellular esterases into the highly water-soluble dye DAF-FM, which traps NO produced by NO synthase (NOS) to yield a highly fluorescent triazole compound in cells (see schematic diagram). Monitoring with a fluorescence microscope should allow direct identification of intracellular production and location of NO.

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