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The distribution of neurofibrils coextensive with microtubules and neurofilaments in dendrites and axons of the tectum, cerebellum, and pallium of the frog

1971; Wiley; Volume: 143; Issue: 4 Linguagem: Inglês

10.1002/cne.901430402

1096-9861

Heather D. Potter,

Barrier Structure and Function Studies

Abstract Correlation of electron micrographs with the results of a variety of reduced silver stains for light microscopy indicates that neurofibrils may be stained in locations where either neurofilaments or microtubules predominate, depending upon the procedure. Reduced silver techniques following fixation with alcohol or aldehyde solutions (Cajal's block method II, methods of Nonidez, Bartelmez, DeCastro, Bodian, Guillery, and Holmes) stain neurofibrils coextensively with neurofilaments (fnf) in myelinated axons, large dendrites, and synaptic boutons but do not stain neurofibrils in most radial dendrites of the tectum or parallel fibers of the cerebellum, where numerous microtubules but few neurofilaments are present. In the latter locations neurofibrils (tnf) are stained following primary fixation in silver nitrate alone (Cajal's block method I) or alcoholic silver nitrate (method Ic), with gold toning of the sections. The methods staining tnf are highly successful in showing the distribution and orientation of the parallel fibers and radial dendrites as well as other thin axons and dendrites in the grey matter including the archipallium. Even in regions containing fnf in numerous processes, it is clear that the majority of fibers in the adult frog brain contain only tnf .