Portal de Periódicos da CAPES

Production of a New Thiopeptide Antibiotic, TP-1161, by a Marine Nocardiopsis Species

2010; American Society for Microbiology; Volume: 76; Issue: 15 Linguagem: Inglês

10.1128/aem.00741-10

1098-5336

Kerstin Engelhardt, Kristin F. Degnes, Michael Kemmler, Harald Bredholt, Espen Fjærvik, Geir Klinkenberg, Håvard Sletta, Trond E. Ellingsen, Sergey B. Zotchev,

Marine Sponges and Natural Products

ABSTRACT Twenty-seven marine sediment- and sponge-derived actinomycetes with a preference for or dependence on seawater for growth were classified at the genus level using molecular taxonomy. Their potential to produce bioactive secondary metabolites was analyzed by PCR screening for genes involved in polyketide and nonribosomal peptide antibiotic synthesis. Using microwell cultures, conditions for the production of antibacterial and antifungal compounds were identified for 15 of the 27 isolates subjected to this screening. Nine of the 15 active extracts were also active against multiresistant Gram-positive bacterial and/or fungal indicator organisms, including vancomycin-resistant Enterococcus faecium and multidrug-resistant Candida albicans . Activity-guided fractionation of fermentation extracts of isolate TFS65-07, showing strong antibacterial activity and classified as a Nocardiopsis species, allowed the identification and purification of the active compound. Structure elucidation revealed this compound to be a new thiopeptide antibiotic with a rare aminoacetone moiety. The in vitro antibacterial activity of this thiopeptide, designated TP-1161, against a panel of bacterial strains was determined.