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Implication of the Lymphocyte-Specific Nuclear Body Protein Sp140 in an Innate Response to Human Immunodeficiency Virus Type 1

2002; American Society for Microbiology; Volume: 76; Issue: 21 Linguagem: Inglês

10.1128/jvi.76.21.11133-11138.2002

1098-5514

Navid Madani, Robert L. Millette, Emily J. Platt, Mariana Marin, Susan L. Kozak, Donald B. Bloch, David Kabat,

HIV/AIDS drug development and treatment

ABSTRACT The viral infectivity factor (Vif) of human immunodeficiency virus type 1 (HIV-1) neutralizes an unidentified antiviral pathway that occurs only in nonpermissive (NP) cells. Using a yeast two-hybrid screen of a human lymphocyte cDNA library, we identified several potential Vif partners. One, the nuclear body protein Sp140, was found specifically in all NP cells ( n = 12 cell lines tested; P ≤ 0.001), and HIV-1 infection induced its partial dispersal from nuclear bodies into cytosolic colocalization with Vif. Our results implicate Sp140 in a response to HIV-1 that may be related to or coordinated with the pathway that inactivates HIV-1 lacking vif .