Molecularly imprinted solid-phase extraction of cocaine metabolites from aqueous samples
2005; Elsevier BV; Volume: 542; Issue: 1 Linguagem: Inglês
10.1016/j.aca.2004.12.019
ISSN1873-4324
AutoresAmaia Zurutuza, Sami Bayoudh, Peter A. G. Cormack, Laurent Dambies, Joseph Deere, Rainer Bischoff, David C. Sherrington,
Tópico(s)Mass Spectrometry Techniques and Applications
ResumoCocaine is a well-known drug of abuse which, when ingested nasally or by smoking, undergoes a number of biotransformation and degradation reactions. In the present work, a synthetic analogue of the cocaine metabolite benzoylecgonine was prepared and used as a template molecule in the preparation of a series of molecularly imprinted polymers (MIPs). Molecularly imprinted solid-phase extraction (MISPE) conditions were established under which benzoylecgonine in aqueous samples could be selectively extracted and quantified at clinically relevant concentrations (μg/ml). Under optimised MISPE conditions, recoveries of analyte were high (>70%) and excellent discrimination between imprinted and non-imprinted materials observed.
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