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Bioorthogonal Imaging of Aurora Kinase A in Live Cells

2012; Wiley; Volume: 51; Issue: 27 Linguagem: Inglês

10.1002/anie.201200994

1521-3773

Katherine S. Yang, Ghyslain Budin, Thomas Reiner, Claudio Vinegoni, Ralph Weissleder,

Photosynthetic Processes and Mechanisms

In living color: Aurora kinase A (AKA) was imaged in live cells using a bioorthogonal two-step reaction with a small molecule AKA inhibitor (see scheme) and a fluorescent reporter. The fluorescent molecule was localized to spindle poles and microtubules during metaphase, consistent with the localization of both endogenous and green fluorescent protein tagged AKA. By using this approach, changes in AKA distribution during mitosis were also observed. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.