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Protein Profiling of Microdissected Pancreas Carcinoma and Identification of HSP27 as a Potential Serum Marker

2007; American Association for Clinical Chemistry; Volume: 53; Issue: 4 Linguagem: Inglês

10.1373/clinchem.2006.079194

1530-8561

Christian Melle, Günther Ernst, Niko Escher, Daniel Hartmann, Bettina Schimmel, Annett Bleul, Heike Thieme, Roland Kaufmann, Klaus Felix, Helmut Frieß, Utz Settmacher, Merten Hommann, Konrad Klaus Richter, Wolfgang Daffner, Horst Täubig, Thomas Manger, Uwe Claussen, Ferdinand von Eggeling,

Endoplasmic Reticulum Stress and Disease

Abstract Background: Patients with pancreatic adenocarcinomas have a poor prognosis because of late clinical manifestation and the tumor’s aggressive nature. We used proteomic techniques to search for markers of pancreatic carcinoma. Methods: We performed protein profiling of microdissected cryostat sections of 9 pancreatic adenocarcinomas and 10 healthy pancreatic tissue samples using ProteinChip technology (surface-enhanced laser desorption/ionization). We identified proteins by use of 2-dimensional gel electrophoresis, peptide fingerprint mapping, and immunodepletion and used immunohistochemistry for in situ localization of the proteins found. We used ELISA to quantify these proteins in preoperative serum samples from 35 patients with pancreatic cancer and 37 healthy individuals. Results: From among the differentially expressed signals that were detected by ProteinChip technology, we identified 2 proteins, DJ-1 and heat shock protein 27 (HSP27). We then detected HSP27 in sera of patients by use of ELISA, indicating a sensitivity of 100% and a specificity of 84% for the recognition of pancreatic cancer. Conclusions: The detection of DJ-1 and HSP27 in pure defined tissue and the retrieval of HSP27 in serum by antibody-based methods identifies a potential marker for pancreatic cancer.