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Identification of murine endogenous xenotropic retrovirus in cultured multicellular tumour spheroids from nude‐mouse‐passaged nasopharyngeal carcinoma

1979; Wiley; Volume: 23; Issue: 1 Linguagem: Inglês

10.1002/ijc.2910230102

1097-0215

Dorothy H. Crawford, B. G. Achono, Natalie M. Teich, S. Finerty, J. L. Thompson, M. A. Epstein, Beppino C. Giovanella,

Viral-associated cancers and disorders

Abstract After nude‐mouse‐passage, a carcinoma of the nasopharynx was found to contain a few scattered C‐t/pe retrovirus particles. Culture of this nude‐mouse‐grown material over solid agar allowed the human tumour cells to grow as multicellular spheroids which increased in number by budding and could be subcultured for up to 6 months. Within the spheroids the human tumour cells expressed increased retrovirus replication and large numbers of C‐type particles were observed. Treatment with a halogenated pyrimidine further enhanced the virus production. This tissue culture system provided sufficient virus production for the identification of the retrovirus as an endogenous xenotropic murine leukaemia virus and not a human agent.