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Occurrence of the Parasite Genus Hematodinium (Alveolata: Syndinea) in the Water Column

2011; Wiley; Volume: 58; Issue: 5 Linguagem: Inglês

10.1111/j.1550-7408.2011.00570.x

1550-7408

Kristina M. Hamilton, Ian Tew, Roger Atkinson, E. C. Roberts,

Marine Ecology and Invasive Species

Journal of Eukaryotic MicrobiologyVolume 58, Issue 5 p. 446-451 Occurrence of the Parasite Genus Hematodinium (Alveolata: Syndinea) in the Water Column KRISTINA M. HAMILTON, KRISTINA M. HAMILTON Department of Biosciences, Swansea University, Singleton Park, Swansea SA2 8PP, United Kingdom School of Biosciences, Geoffrey Pope Building, University of Exeter, Devon, United KingdomSearch for more papers by this authorIAN F. TEW, IAN F. TEW Department of Biosciences, Swansea University, Singleton Park, Swansea SA2 8PP, United KingdomSearch for more papers by this authorR. JIM A. ATKINSON, R. JIM A. ATKINSON University Marine Biological Station, Millport, Great Cumbrae, Scotland KA28 0EG, United KingdomSearch for more papers by this authorEMILY C. ROBERTS, EMILY C. ROBERTS Department of Biosciences, Swansea University, Singleton Park, Swansea SA2 8PP, United KingdomSearch for more papers by this author KRISTINA M. HAMILTON, KRISTINA M. HAMILTON Department of Biosciences, Swansea University, Singleton Park, Swansea SA2 8PP, United Kingdom School of Biosciences, Geoffrey Pope Building, University of Exeter, Devon, United KingdomSearch for more papers by this authorIAN F. TEW, IAN F. TEW Department of Biosciences, Swansea University, Singleton Park, Swansea SA2 8PP, United KingdomSearch for more papers by this authorR. JIM A. ATKINSON, R. JIM A. ATKINSON University Marine Biological Station, Millport, Great Cumbrae, Scotland KA28 0EG, United KingdomSearch for more papers by this authorEMILY C. ROBERTS, EMILY C. ROBERTS Department of Biosciences, Swansea University, Singleton Park, Swansea SA2 8PP, United KingdomSearch for more papers by this author First published: 18 August 2011 https://doi.org/10.1111/j.1550-7408.2011.00570.xCitations: 10 Corresponding Author: E.C. Roberts, Department of Biosciences, Swansea University, Singleton Park, Swansea SA2 8PP, United Kingdom—Telephone number: +44 1792 513002; FAX number: +44 1792 295447; e-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract ABSTRACT. Crustaceans worldwide are infected with alveolate parasites of the genus Hematodinium, causing substantial losses to langoustine and crab fisheries. The distinct seasonality in Hematodinium occurrence in their decapod hosts, as well as unsuccessful attempts at transmission, suggest the existence of life stages outside their benthic crustacean hosts. We used a nested polymerase chain reaction method to detect Hematodinium rDNA in the environment and in potential alternative hosts. Environmental samples from the Clyde Sea, Scotland, were screened during the April release of dinospores and during June and August, when infection prevalence is rare in benthic crustaceans. Hematodinium rDNA was amplified in 15% (14/94) of isolated langoustine larvae, and in 12% (13/111) of crab larvae. In addition, Hematodinium rDNA was present in mixed plankton samples devoid of decapod larvae, but including the 2 μm–10 mm fraction of particulate organic matter in the water column, containing phytoplankton and other zooplankton. These results indicate that Hematodinium occurs in the water column and is harboured by planktonic organisms, including larval stages of the crustacean hosts, when infections are at their lowest in adult hosts. Supporting Information Fig. S1. The Firth of Clyde on the Scottish west coast indicating the two sampling sites. SK: Skelmorlie (55° 51′ N, 4° 54′ W); MC: Main Channel (55° 45′ N, 4° 58′ W). Table S1. Sampling data for the two sites in the Clyde Sea, Scotland (MC: main channel; SK: Skelmorlie). Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Filename Description JEU_570_sm_supplfig-s1.tif5.4 MB Supporting info item JEU_570_sm_suppltable-s1.doc44.5 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. LITERATURE CITED Appleton, P. L. & Vickerman, K. 1998. In vitro cultivation and developmental cycle in culture of a parasitic dinoflagellate (Hematodinium sp.) associated with mortality of the Norway lobster (Nephrops norvegicus) in British waters. Parasitology, 116: 115–130. Audermard, C., Le Roux, F., Barnaud, A., Collins, C., Sautour, B., Sauriau, R. G., de Montaudouin, X., Coustau, C., Combes, C. & Berthe, F. 2002. Needle in a haystack: involvement of the copepod Paracartia grani in the life-cycle of the oyster pathogen Marteilia refringens. Parasitology, 124: 315–323. Becnel, J. J. & Andreadis, T. G. 1998. 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