Marchantin C, a macrocyclic bisbibenzyl, induces apoptosis of human glioma A172 cells
2008; Elsevier BV; Volume: 262; Issue: 2 Linguagem: Inglês
10.1016/j.canlet.2007.12.014
ISSN1872-7980
AutoresYan‐Qiu Shi, Yongxiang Liao, Xian‐Jun Qu, Huiqing Yuan, Song Li, Jian‐Bo Qu, Hong‐Xiang Lou,
Tópico(s)Phytochemical compounds biological activities
ResumoMacrocyclic bisbibenzyls, a class of characteristic components derived from liverworts, are attracting more and more attention because of their wide range of biological significance, including anti-bacterial, anti-fungus, anti-oxidation and cytotoxicity. Herein, we investigated the pro-apoptotic effect of marchantin C on human glioma A 172 cells. The results demonstrated that marchantin C conferred dose-dependent inhibitory effects onto cell growth, viability and colony formation ability of A 172 cells. Morphological observation and DNA laddering assay showed that marchantin C-treated A172 cells displayed outstanding apoptosis characteristics, such as nuclear fragmentation, the appearance of membrane-enclosed apoptotic bodies and DNA laddering fragment. Moreover, flow cytometric detection of phosphatidylserine externalization indicated that marchantin C-induced apoptosis occurred in a dose-dependent manner. RT-PCR and western blot assay further substantiated that marchantin C, as a promising pro-apoptotic agent, had strong effects to induce A172 cell apoptosis, suggesting that the action was achieved through up-regulating Bax and down-regulating Bcl-2.
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