Portal de Periódicos da CAPES

Tear sample collection using cellulose acetate absorbent filters

2008; Wiley; Volume: 28; Issue: 6 Linguagem: Inglês

10.1111/j.1475-1313.2008.00603.x

1475-1313

Marieh Esmaeelpour, Jun Cai, Patrick Watts, Mike Boulton, Paul J. Murphy,

Advanced Drug Delivery Systems

Abstract Purpose: To assess the use of cellulose acetate filter rods as a technique for tear collection. Method: The cellulose acetate rod (CR) was compared with the ‘standard’ glass capillary tube (CT), in a series of experiments, to assess: sample collection by collected volume size; the effect of tear stimulation on total tear protein concentration and major tear protein concentrations; and technique invasiveness. Results: No difference was found in concentrations for total protein, IgA (secretory immunoglobulin A), lactoferrin and lysozyme ( p > 0.05) with no clinically significant increase in serum albumin to show serum leakage. Sample volume was higher for CR ( p < 0.005) and sample volume increased for stimulated collection with CR ( p = 0.001). Dilution effect of a stimulated sample size was reliably shown only with CR ( r = −0.66, p = 0.011). Using bovine albumin standard with CR and CT, a smaller sample volume ( p < 0.001) and a higher protein concentration ( p < 0.001) were extracted with CR. Conclusion: The cellulose rod offers a suitable alternative to the glass CT. It is able to quickly absorb a sample, allowing use for a wide range of sample sizes, while being minimally invasive.