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Use of polymerase chain reaction to diagnose tuberculous arthritis from joint tissues and synovial fluid.

2004; American Medical Association; Volume: 128; Issue: 2 Linguagem: Inglês

10.1043/1543-2165(2004)128 2.0.co;2

1543-2165

А. Г. Титов, Elena Vyshnevskaya, Sergei I. Mazurenko, Seppo Santavirta, Yrjö T. Konttinen,

Mycobacterium research and diagnosis

Abstract Context.—Tuberculosis of the joints and bones is a significant worldwide problem, often leading to joint and bone destruction. The diagnosis of this disease manifestation is difficult. Objective.—To assess the role of conventional diagnostics compared to polymerase chain reaction applied to samples obtained at arthroscopy. Design.—This was an open observational study that was blinded to the microbiologist, histopathologist, and molecular biologist responsible for assessing the main outcome measures. Patients.—Seven patients (8 samples) with joint and bone tuberculosis and 14 patients (16 samples) with nontuberculous joint and bone disease. Intervention.—Arthroscopic examination and tissue sample collection. Main Outcome Measures.—Mycobacterium tuberculosis staining, culture, and histopathologic assessment of caseating granulomas vs polymerase chain reaction. Results.—Polymerase chain reaction was positive in all cases of true tuberculosis and falsely identified 2 samples as positive, both however...