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Methodological Characterization of the 2‐Keto [1‐ 13 C]isocaproate Breath Test to Measure in Vivo Human Mitochondrial Function: Application in Alcoholic Liver Disease Assessment

2003; Wiley; Volume: 27; Issue: 8 Linguagem: Inglês

10.1097/01.alc.0000081623.25175.7e

1530-0277

Dolores Aleixandre Parra, Álvaro González, Luis García‐Villarreal, J. Alfredo Martínéz,

Metabolism and Genetic Disorders

Background: The 2‐keto[1‐ 13 C]isocaproate oxidation measurement has been shown as a helpful tool in the in vivo assessment of liver mitochondrial function. Methods: The aim of this work was to study the variability of the 2‐keto[1‐ 13 C]isocaproate breath test in 24 healthy controls (8 men and 16 women) and to evaluate its clinical usefulness in 20 patients (14 men and 6 women) with liver disease (7 men with history of alcoholism). Breath test was performed by measuring 13 CO 2 enrichment in breath before and after the oral administration of the tracer and by using isotope ratio mass spectrometry. Results: The intrasubject and intersubject variability of the percentage of tracer oxidized were 8 and 14%, respectively. The 2‐keto[1‐ 13 C]isocaproate oxidation in women was faster ( p = 0.004) and tended to be higher ( p = 0.050) than in men. The percentage of oxidized tracer was lower in those patients with alcoholic liver disease than in healthy volunteers ( p = 0.001) and in nonalcoholic patients ( p = 0.003). Conclusions: The percentage of tracer oxidized appears as a convenient parameter to detect impairment in liver mitochondrial oxidation related to alcoholism by the 2‐keto[1‐ 13 C]isocaproate breath test, establishing different cutoff values depending on gender.