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Chronic exposure to nanosized, anatase titanium dioxide is not cyto- or genotoxic to Chinese hamster ovary cells

2011; Wiley; Volume: 52; Issue: 8 Linguagem: Inglês

10.1002/em.20660

1098-2280

Shuguang Wang, Lindsey Hunter, Zikri Arslan, Michael G. Wilkerson, Jeffrey K. Wickliffe,

Healthcare and Environmental Waste Management

Environmental and Molecular MutagenesisVolume 52, Issue 8 p. 614-622 Research Article Chronic exposure to nanosized, anatase titanium dioxide is not cyto- or genotoxic to Chinese hamster ovary cells Shuguang Wang, Shuguang Wang Department of Environmental Health Sciences, Tulane University, New Orleans, LouisianaSearch for more papers by this authorLindsey A. Hunter, Lindsey A. Hunter Department of Dermatology, School of Medicine, University of Texas Medical Branch, Galveston, TexasSearch for more papers by this authorZikri Arslan, Zikri Arslan Department of Chemistry and Biochemistry, Jackson State University, Jackson, MississippiSearch for more papers by this authorMichael G. Wilkerson, Michael G. Wilkerson Department of Dermatology, School of Medicine, University of Texas Medical Branch, Galveston, TexasSearch for more papers by this authorJeffrey K. Wickliffe, Corresponding Author Jeffrey K. Wickliffe [email protected] Department of Environmental Health Sciences, Tulane University, New Orleans, LouisianaTulane University School of Public Health and Tropical Medicine, Department of Environmental Health Sciences, 1440 Canal Street, Suite 2100, New Orleans, LA 70112Search for more papers by this author Shuguang Wang, Shuguang Wang Department of Environmental Health Sciences, Tulane University, New Orleans, LouisianaSearch for more papers by this authorLindsey A. Hunter, Lindsey A. Hunter Department of Dermatology, School of Medicine, University of Texas Medical Branch, Galveston, TexasSearch for more papers by this authorZikri Arslan, Zikri Arslan Department of Chemistry and Biochemistry, Jackson State University, Jackson, MississippiSearch for more papers by this authorMichael G. Wilkerson, Michael G. Wilkerson Department of Dermatology, School of Medicine, University of Texas Medical Branch, Galveston, TexasSearch for more papers by this authorJeffrey K. Wickliffe, Corresponding Author Jeffrey K. Wickliffe [email protected] Department of Environmental Health Sciences, Tulane University, New Orleans, LouisianaTulane University School of Public Health and Tropical Medicine, Department of Environmental Health Sciences, 1440 Canal Street, Suite 2100, New Orleans, LA 70112Search for more papers by this author First published: 22 July 2011 https://doi.org/10.1002/em.20660Citations: 40Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Titanium dioxide nanoparticles (nano-TiO2) are widely used in cosmetics, skin care products, paints, and water treatment processes. Disagreement remains regarding the safety of nano-TiO2, and little epidemiological data is available to provide needed resolution. Most studies have examined effects using acute exposure experiments with relatively few studies using a chronic exposure design. We examined cyto- and genotoxicity in CHO-K1 cells following 60 days of continuous exposure to defined levels of nano-TiO2 (0, 10, 20, or 40 μg/ml). Oxidative stress increased in a concentration-dependent manner in short- (2 days) and long-term cultures, but long-term cultures had lower levels of oxidative stress. The primary reactive oxygen species appeared to be superoxide, and ROS indicators were lowered with the addition of superoxide dismutase (SOD). No cyto- or genotoxic effects were apparent using the XTT, trypan-blue exclusion, and colony-forming assays for viability and the Comet and Hprt gene mutation assays for genotoxicity. Nano-TiO2 increased the percentage of cells in the G2/M phase of the cell cycle, but this effect did not appear to influence cell viability or cell division. Cellular Ti content was dose-dependent, but chronically exposed cells had lower amounts than acutely exposed cells. CHO cells appear to adapt to chronic exposure to nano-TiO2 and detoxify excess ROS possibly through upregulation of SOD in addition to reducing particle uptake. Environ. Mol. Mutagen. 2011. © 2011 Wiley-Liss, Inc. Citing Literature Volume52, Issue8October 2011Pages 614-622 RelatedInformation