Portal de Periódicos da CAPES

CD45R, CD44 and MHC class II are signaling molecules for the cytoskeleton-dependent induction of dendrites and motility in activated B cells

2000; Wiley; Volume: 30; Issue: 9 Linguagem: Inglês

10.1002/1521-4141(200009)30

1521-4141

Santiago Partida‐Sánchez, Adriana Garibay‐Escobar, Eugenio Frixione, R. M. E. Parkhouse, Leopoldo Santos‐Argumedo,

T-cell and B-cell Immunology

European Journal of ImmunologyVolume 30, Issue 9 p. 2723-2728 ArticleFree Access CD45R, CD44 and MHC class II are signaling molecules for the cytoskeleton-dependent induction of dendrites and motility in activated B cells Santiago Partida-Sánchez, Santiago Partida-Sánchez Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoSearch for more papers by this authorAdriana Garibay-Escobar, Adriana Garibay-Escobar Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoSearch for more papers by this authorEugenio Frixione, Eugenio Frixione Department of Cell Biology, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoSearch for more papers by this authorR. M. E. Parkhouse, R. M. E. Parkhouse Department of Immunology, Instituto Gulbenkian de Ciencia, Oeiras, PortugalSearch for more papers by this authorLeopoldo Santos-Argumedo, Corresponding Author Leopoldo Santos-Argumedo lesantos@mail.cinvestav.mx Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoDepartment of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., Apartado Postal 14–740, cp 07360, México D.F., Mexico, Fax: +525-747-7134 & 747–7002Search for more papers by this author Santiago Partida-Sánchez, Santiago Partida-Sánchez Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoSearch for more papers by this authorAdriana Garibay-Escobar, Adriana Garibay-Escobar Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoSearch for more papers by this authorEugenio Frixione, Eugenio Frixione Department of Cell Biology, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoSearch for more papers by this authorR. M. E. Parkhouse, R. M. E. Parkhouse Department of Immunology, Instituto Gulbenkian de Ciencia, Oeiras, PortugalSearch for more papers by this authorLeopoldo Santos-Argumedo, Corresponding Author Leopoldo Santos-Argumedo lesantos@mail.cinvestav.mx Department of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., México D.F., MéxicoDepartment of Molecular Biomedicine, Centro de Investigación y Estudios Avanzados del I.P.N., Apartado Postal 14–740, cp 07360, México D.F., Mexico, Fax: +525-747-7134 & 747–7002Search for more papers by this author First published: 24 August 2000 https://doi.org/10.1002/1521-4141(200009)30:9 3.0.CO;2-5Citations: 7AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Anti-CD44 or anti-MHC II antibodies bound to tissue culture plates have previously been shown to induce a dramatic generation of dendritic processes in activated murine B cells. In this study, we demonstrate a similar generation of dendrites and cell motility in activated B cells through CD45R. The dynamic formation of dendritic processes and associated induction of cell motility were analyzed by video microscopy and were characterized by a rapid, and multidirectional emission of dendrites with retractile behavior. The addition of cytochalasin E totally blocked dendrites formation and motility induced through either CD45R, CD44 or MHC II, suggesting that the necessary cytoskeletal rearrangements require active polymerization of actin. Confocal microscopy showed an accumulation of F-actin in the dendrites, as long as cells were elongating. In contrast, G-actin was localized in the perinuclear area and also accumulated in sites where dendrites originated. Preincubation of B cells with staurosporine (a PKC inhibitor) or BAPTA-AM (a calcium chelator) prevented these morphological changes, indicating additionally a requirement for a PKC-calcium-dependent activity. Dendrite formation and cellular motility, therefore, seem to be two manifestations of the same phenomenon, and CD44, CD45R and MHC II appear to be signaling molecules for the observed cytoskeleton-dependent morphological changes. Citing Literature Volume30, Issue9September 2000Pages 2723-2728 RelatedInformation