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Determination of Trimethoprim-Sulfamethoxazole Resistance in Streptococcus pneumoniae by Using the E Test with Mueller-Hinton Agar Supplemented with Sheep or Horse Blood May Be Unreliable

1999; American Society for Microbiology; Volume: 37; Issue: 1 Linguagem: Inglês

10.1128/jcm.37.1.215-217.1999

1098-660X

Marguerite Lovgren, Lillia Dell'Acqua, Rosario Palacio, Gabriela Echániz‐Aviles, Araceli Soto‐Noguerón, Elizabeth Castañeda, Clara Inés Agudelo, Ingrid Heitmann, Maria Cristina de Cunto Brandileone, Rosemeire Cobo Zanella, Alicia Rossi, Julio Pace, James Talbot,

Antibiotics Pharmacokinetics and Efficacy

ABSTRACT An international, multicenter study compared trimethoprim-sulfamethoxazole MICs for 743 Streptococcus pneumoniae isolates (107 to 244 isolates per country) by E test, using Mueller-Hinton agar supplemented with 5% defibrinated horse blood or 5% defibrinated sheep blood, with MICs determined by the National Committee for Clinical Laboratory Standards broth microdilution reference method. Agreement within 1 log 2 dilution and minor error rates were 69.3 and 15.5%, respectively, on sheep blood-supplemented agar and 76.9 and 13.6%, respectively, with horse blood as the supplement. Significant interlaboratory variability was observed. E test may not be a reliable method for determining the resistance of pneumococci to trimethoprim-sulfamethoxazole.