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High-Affinity Peptide Ligands to Prostate-Specific Antigen Identified by Polysome Selection

1997; Elsevier BV; Volume: 232; Issue: 2 Linguagem: Inglês

10.1006/bbrc.1997.6331

1090-2104

Geoffrey M. Gersuk, Michael J. Corey, Eva Corey, James E. Stray, G Kawasaki, Robert L. Vessella,

vaccines and immunoinformatics approaches

We have used the polysome-selection method to isolate peptide ligands that bind with high affinity to Prostate-Specific Antigen (PSA), an important prostate-cancer marker. Two random libraries, each encoding approximately 1012random peptides, were transcribed into RNA and translatedin vitro.Polysomes were panned by affinity selection of the nascent peptides against immobilized PSA. Over 30% of the selected species had significant affinity for PSA; the dissociation constant of the complex formed by the best isolate with PSA was <10−9M. Formation of streptavidin conjugates of selected peptides improved their affinities and, in one case, virtually eliminated non-specific binding. The polysome-selection method can be used to produce high-affinity peptide ligands of potential use in diagnostic and therapeutic procedures.