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Mapping and identification of essential gene functions on the X chromosome of Drosophila

2002; Springer Nature; Volume: 3; Issue: 1 Linguagem: Inglês

10.1093/embo-reports/kvf012

1469-3178

Annette Peter, Petra Schöttler, Meike Werner, Nicole Beinert, Gordon Dowe, P. Burkert, Foteini Mourkioti, Lore Dentzer, Yuchun He, Péter Deák, Panayiotis V. Benos, Melanie K. Gatt, Lee Murphy, D. James Harris, Bart Barrell, Concepción Ferraz, Sophie Vidal, Christine Brun, Jacques Demaille, Édouard Cadieu, Stéphane Dréano, Stéphanie Gloux, Valérie Lelaure, Stéphanie Mottier, Francis Galibert, Dana Borkova, Belén Miñana, Fotis C. Kafatos, Slava Bolshakov, Inga Sidén‐Kiamos, George Papagiannakis, Lefteris Spanos, Christos Louis, Encarnación Madueño, Beatriz de Pablos, Juan Modolell, Alain Bucheton, Debbie Callister, Lorna Campbell, Nadine S. Henderson, Paul J. McMillan, Cathy Salles, Evelyn Tait, Phillipe Valenti, Robert D. C. Saunders, Alain Billaud, Lior Pachter, Robert Klapper, Wilfried Janning, David M. Glover, Michael Ashburner, Hugo J. Bellen, Herbert Jäckle, Ulrich Schäfer,

Insect Resistance and Genetics

The Drosophila melanogaster genome consists of four chromosomes that contain 165 Mb of DNA, 120 Mb of which are euchromatic. The two Drosophila Genome Projects, in collaboration with Celera Genomics Systems, have sequenced the genome, complementing the previously established physical and genetic maps. In addition, the Berkeley Drosophila Genome Project has undertaken large‐scale functional analysis based on mutagenesis by transposable P element insertions into autosomes. Here, we present a large‐scale P element insertion screen for vital gene functions and a BAC tiling map for the X chromosome. A collection of 501 X‐chromosomal P element insertion lines was used to map essential genes cytogenetically and to establish short sequence tags (STSs) linking the insertion sites to the genome. The distribution of the P element integration sites, the identified genes and transcription units as well as the expression patterns of the P‐element‐tagged enhancers is described and discussed.