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Two-photon fluorescence cross-section measurements calibrated with hyper-Rayleigh scattering

1999; Optica Publishing Group; Volume: 16; Issue: 6 Linguagem: Inglês

10.1364/josab.16.000998

1520-8540

P. Kaatz, David P. Shelton,

Photoacoustic and Ultrasonic Imaging

The intensity of two-photon-excited fluorescence measured relative to the intensity of hyper-Rayleigh scattering in the same apparatus was used to determine the two-photon-excited fluorescence cross sections of the xanthene dyes Rhodamine B, Rhodamine 6G, and Fluorescein. The measured cross sections at λ0=1064 nm are σ2PF(2)=1.5±0.3×10-50 cm4 s,2.0±0.4×10-50 cm4 s, and 1.4±0.3×10-52 cm4 s, for the three dyes, respectively. The two-photon-excited fluorescence cross section of Rhodamine B was also calibrated with one-photon-excited fluorescence in this apparatus. The result, σ2PF(2)=2.3±0.7×10-50 cm4 s, is in reasonable agreement with the result calibrated by hyper-Rayleigh scattering. The results for Rhodamine B are 1.5–6 times smaller than previous results calibrated by one-photon-excited fluorescence.