Molecular analysis to demonstrate that odontogenic keratocysts are neoplastic.
2004; American Medical Association; Volume: 128; Issue: 3 Linguagem: Inglês
10.1043/1543-2165(2004)128 2.0.co;2
ISSN1543-2165
AutoresNarasimhan P. Agaram, Bobby Collins, Leon Barnes, Deren Lomago, Dalal Aldeeb, Patricia A. Swalsky, Sydney Finkelstein, Jennifer L. Hunt,
Tópico(s)Bone Tumor Diagnosis and Treatments
ResumoAbstract Context.—Odontogenic keratocysts (OKCs) are unique odontogenic lesions that have the potential to behave aggressively, that can recur, and that can be associated with the nevoid basal cell carcinoma syndrome. Whether they are developmental or neoplastic continues to be debated. Objectives.—To identify loss of heterozygosity of tumor suppressor genes in OKCs and to suggest a pathogenetic origin for these lesions. Design.—We examined 10 OKCs for loss of heterozygosity of tumor suppressor genes, using a microdissection and semiquantitative genotyping analysis. The genes analyzed included 10 common tumor suppressor genes, as well as the PTCH gene, which is mutated in nevoid basal cell carcinoma syndrome. Results.—Loss of heterozygosity was seen in 7 of 10 cases, with a frequency between 11% and 80% of the genes studied. The genes that exhibited the most frequent allelic losses were p16, p53, PTCH, and MCC (75%, 66%, 60%, and 60%, respectively). Daughter cysts were associated with a higher frequency o...
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