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Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

2004; Royal Society of Chemistry; Issue: 20 Linguagem: Inglês

10.1039/b411973h

1364-548X

Russell J. Cox, Frank Glod, Deirdre Hurley, Colin M. Lazarus, Thomas P. Nicholson, Brian A.M. Rudd, Thomas J. Simpson, Barrie Wilkinson, Ying Zhang,

Plant biochemistry and biosynthesis

PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the biosynthesis of the squalestatin side-chain.