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Comprehensive and innovative techniques for liver transplantation in rats: A surgical guide

2010; Baishideng Publishing Group; Volume: 16; Issue: 25 Linguagem: Inglês

10.3748/wjg.v16.i25.3120

2219-2840

Tomohide Hori, Justin H. Nguyen, Xiangdong Zhao, Yasuhiro Ogura, Toshiyuki Hata, Shintaro Yagi, Feng Chen, Ann-Marie T. Baine, Norifumi Ohashi, Christopher B. Eckman, Aimee Herdt, Hiroto Egawa, Yasutsugu Takada, Fumitaka Oike, Seisuke Sakamoto, Mureo Kasahara, Kohei Ogawa, Koichiro Hata, Taku Iida, Yukihide Yonekawa, Lena Sibulesky, Kagemasa Kuribayashi, Takuma Kato, Kanako Saito, Linan Wang, Mie Torii, Naruhiko Sahara, Naoko Kamo, Tomoko Sahara, Motohiko Yasutomi, Shinji Üemoto,

Liver physiology and pathology

To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery.We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis.OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model.