Protection from cytosolic prion protein toxicity by modulation of protein translocation
2004; Springer Nature; Volume: 23; Issue: 23 Linguagem: Inglês
10.1038/sj.emboj.7600462
ISSN1460-2075
AutoresNeena S. Rane, Jesse L Yonkovich, Ramanujan S. Hegde,
Tópico(s)Alzheimer's disease research and treatments
ResumoArticle4 November 2004free access Protection from cytosolic prion protein toxicity by modulation of protein translocation Neena S Rane Neena S Rane Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, MD, USA Search for more papers by this author Jesse L Yonkovich Jesse L Yonkovich Search for more papers by this author Ramanujan S Hegde Corresponding Author Ramanujan S Hegde Search for more papers by this author Neena S Rane Neena S Rane Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, MD, USA Search for more papers by this author Jesse L Yonkovich Jesse L Yonkovich Search for more papers by this author Ramanujan S Hegde Corresponding Author Ramanujan S Hegde Search for more papers by this author Author Information Neena S Rane1, Jesse L Yonkovich and Ramanujan S Hegde 1Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, MD, USA *Corresponding author. Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, 18 Library Drive, Building 18T, Room 101, Bethesda, MD 20892-5430, USA. Tel.: +1 301 496 4855; Fax: +1 301 402 0078; E-mail: [email protected] The EMBO Journal (2004)23:4550-4559https://doi.org/10.1038/sj.emboj.7600462 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Failure to promptly dispose of undesirable proteins is associated with numerous diseases. In the case of cellular prion protein (PrP), inhibition of the proteasome pathway can generate a highly aggregation-prone, cytotoxic form of PrP implicated in neurodegeneration. However, the predominant mechanisms that result in delivery of PrP, ordinarily targeted to the secretory pathway, to cytosolic proteasomes have been unclear. By accurately measuring the in vivo fidelity of protein translocation into the endoplasmic reticulum (ER), we reveal a slight inefficiency in PrP signal sequence function that generates proteasomally degraded cytosolic PrP. Attenuating this source of cytosolic PrP completely eliminates the dependence on proteasomes for PrP degradation. This allows cells to tolerate both higher expression levels and decreased proteasomal capacity without succumbing to the adverse consequences of misfolded PrP. Thus, the generation of potentially toxic cytosolic PrP is controlled primarily during its initial translocation into the ER. These results suggest that a substantial proportion of the cell's constitutive proteasomal burden may consist of proteins that, like PrP, fail to cotranslationally enter the secretory pathway with high fidelity. Introduction The mammalian prion protein (PrP) is a cell surface glycoprotein whose misfolding is associated with both the transmission and pathogenesis of a variety of neurodegenerative diseases (reviewed by Prusiner, 2001; Aguzzi and Haass, 2003). These diseases, which include Creutzfeldt–Jakob disease (CJD) and Gerstmann–Straussler–Scheinker (GSS) disease in humans, are characterized by a progressive spongiform degeneration of neurons accompanied in many cases by the deposition of PrP-containing aggregates in selected regions of the brain. In most, but not all instances of neurodegeneration associated with PrP, the disease is transmissible. The transmissible agent is predominantly composed of a misfolded conformer of PrP termed PrPSc. While the identification of the transmissible agent and its mechanism of propagation have been the subjects of intense study, relatively little is understood about how changes in PrP folding and/or metabolism can lead to neuronal dysfunction and death. Increasingly however, it is becoming clear that the accumulation of PrPSc in the brain, while central to transmission of disease, may not directly cause neurodegeneration. For example, extensive deposition of PrPSc in the context of neurons not actively expressing PrP does not lead to neuronal damage and death (Brandner et al, 1996; Mallucci et al, 2003). Conversely, certain naturally occurring or artificially created mutations in PrP can lead directly to neurodegeneration without generating the transmissible PrPSc form (Tateishi and Kitamoto, 1995; Tateishi et al, 1996; Hegde et al, 1998, 1999). Thus, PrPSc accumulation is neither uniformly necessary nor sufficient for neuronal toxicity. These and other findings have led to the idea that other facets of normal PrP metabolism may play critical roles in disease pathogenesis (Hegde and Lingappa, 1999; Chiesa and Harris, 2001; Hegde and Rane, 2003). Recent studies have begun to implicate events during the initial biogenesis, trafficking, and degradation of PrP at the endoplasmic reticulum (ER) in the development of neurodegeneration (reviewed in Hegde and Rane, 2003). One neurotoxic form of PrP is a transmembrane isoform (termed CtmPrP; Hegde et al, 1998) that is generated during the initial translocation of PrP into the ER (Kim and Hegde, 2002). Although wild-type PrP generates relatively little (<10%) CtmPrP in vivo, several mutations in the potential transmembrane domain significantly increase its generation (Hegde et al, 1998, 1999; Kim et al, 2001; Stewart and Harris, 2001; Kim and Hegde, 2002). Expression of such CtmPrP-favoring mutants in transgenic mice causes neurodegeneration (Hegde et al, 1998, 1999), and at least three naturally occurring human mutations in the transmembrane domain result in increased CtmPrP generation (Hegde et al, 1998; Kim and Hegde, 2002). Thus, CtmPrP appears to play a direct role in at least a subset of PrP-mediated diseases, although the mechanism by which it causes cell death remains unknown. More recently, it has been demonstrated that inappropriate expression of PrP in the cytosol can be both neurotoxic in mice (Ma et al, 2002) and aggregation-prone in cells (Yedidia et al, 2001; Ma and Lindquist, 2002). However, the pathways by which PrP would appear in the cytosol remain speculative and contentious (Ma and Lindquist, 2001, 2002; Yedidia et al, 2001; Drisaldi et al, 2003; Hegde and Rane, 2003; Roucou et al, 2003). In one model, a proportion of PrP is proposed to misfold in the ER lumen, be recognized by the ER quality control machinery, and subsequently be retrotranslocated to the cytosol for degradation by proteasomes (Ma and Lindquist, 2001, 2002). However, the observation that at least some of the PrP that accumulates upon proteasome inhibition contains an uncleaved signal peptide suggests that another route to the cytosol may involve a failure of PrP translocation into the ER (Drisaldi et al, 2003). In either case, the role of cytosolic PrP in disease pathogenesis remains unclear, ranging from protective (Roucou et al, 2003) to toxic roles (Ma et al, 2002), having been proposed by different investigators. Clearly, any putative roles for cytosolic PrP in normal physiology (Roucou et al, 2003) or disease pathogenesis (Yedidia et al, 2001; Ma et al, 2002; Ma and Lindquist, 2002) cannot be delineated without the ability to selectively modulate its generation. This requires an understanding of the pathway(s) by which proteins that are normally trafficked through the secretory pathway can reside in the cytosol. Like most secretory and membrane proteins (Rapoport et al, 1996), PrP contains a signal sequence that mediates cotranslational targeting to and translocation across the ER membrane. The general efficiency of signal-mediated segregation to the ER, particularly in vivo is not well studied. However, in vitro studies have recently suggested that certain early steps in protein translocation across the ER may not be uniformly efficient for all substrates (Rutkowski et al, 2001; Kim et al, 2002). In addition, in vivo analysis of the kinetics of signal-mediated targeting (Johnsson and Varshavsky, 1994; Goder et al, 2000) and the steady-state presence of a signal-containing reporter in the cytosol (Levine et al, 2004) suggest that substrates differ in their efficiency of segregation into the ER. Presumably, the nontranslocated fraction of many such proteins would be viewed as undesirable in the cytosol, and need to be disposed by the ubiquitin–proteasome system. The physiological significance or relative contribution of such inefficiencies in ER protein translocation to the total substrate pool handled by cytosolic proteasomes in vivo has not been examined. In this study, we have analyzed this issue for PrP to reveal an unanticipated relationship between protein translocation, protein degradation, and the potential for PrP aggregation in the cytosol. Results and discussion Measurement of signal sequence efficiencies in vivo A transcription factor (TF) sequestration assay (described in detail elsewhere (Levine et al, 2004); see also Supplementary Figure S1A) was used to measure the in vivo efficiency of signal sequence-mediated protein segregation into the ER. Here, any inefficiency of a signal sequence to direct the TF into the ER is detected by activation of a cotransfected luciferase reporter. When TF lacking a signal sequence was tested, a dose-dependent increase in reporter activity is observed over a broad range of expression (Figure 1A), even at TF levels that are below the detection threshold for immunoblotting (Figure 1B). When TF was preceded by the PrP signal sequence (PrP-TF), reporter activity was reduced sharply (∼10–20-fold) despite identical levels of TF expression (Figure 1A and B). Quantitation relative to the standard curve of signal-less TF suggested that, depending on experimental conditions (e.g., growth state and confluency of the cells), between 5 and 15% of total PrP-TF at steady state (∼15% in Figure 1C) was available in the nucleo-cytoplasmic compartment for reporter activation. Even at very low expression levels in cells growing under optimal conditions, at least 5% of PrP-TF was not segregated into the ER. Figure 1.Measurement and modulation of protein translocation in vivo. (A) Varying amounts of plasmid encoding TF or PrP-TF were transfected into MDCK cells and the amount of luciferase reporter activation measured (in relative light units (RLU)). (B) Duplicate samples from panel A were analyzed in parallel by immunoblotting with antibodies against NF-κB. The position of exogenously expressed TF on faint and dark exposures of the blot is indicated with arrows. Endogenous NF-κB (indicated by an asterisk) serves as a loading control. (C) Comparison of translocation efficiencies of the Opn, PrP, and Prl signal sequences fused to TF. Transfection of an unrelated plasmid (mock) resulted in no reporter activation. Varying amounts of TF lacking a signal sequence were analyzed in parallel (note 10-fold difference in y-axis scales). (D) Parallel immunoblots for TF expression of samples from panel C. Download figure Download PowerPoint When other signal sequences were used in the same assay, the percent of cytoplasmic TF could be substantially increased or decreased relative to PrP-TF (Levine et al, 2004). This is consistent with in vitro observations demonstrating that signal sequences can vary broadly in their functions at early steps in substrate translocation (Rutkowski et al, 2001; Kim et al, 2002). Among the most efficient signal sequences, both in vitro (Kim et al, 2002) and in the TF sequestration assay (Levine et al, 2004; Figure 1C and D and Supplementary Figure S1B), are those from either prolactin (Prl) or osteopontin (Opn). Both of these resulted in ∼50% lower reporter activity relative to PrP-TF at comparable and modest expression levels (Figure 1C and D), indicating that even the highest efficiency signals still permit substantial and readily detectable levels of total TF to exist in a functional state in the nucleo-cytoplasmic compartment. Consequence of signal sequence inefficiency for PrP metabolism To explore the relevance of this small but readily detectable inefficiency in the activity of some signals, we turned our attention to native PrP. Modulating PrP translocation by replacement of its signal sequence with the more efficient Opn or Prl signal sequences (termed Opn-PrP and Prl-PrP, respectively) did not lead to obvious changes in several properties of PrP when examined at steady state in unperturbed cultured cells: the glycosylation pattern, resistance to endoglycosidase H digestion, subcellular localization, solubility in nonionic detergents, surface expression, protease sensitivity, and site of signal sequence cleavage were all indistinguishable between these constructs (Figure 2B and Supplementary Figures S2, S3 and S4 and unpublished results). When analyzed by pulse-chase studies, however, small differences could be detected in the metabolism of these three constructs (Figure 2A). Whereas ∼20% of PrP was observed to be unglycosylated after a brief 15 min pulse labeling, substantially less of this population was observed for Opn-PrP and Prl-PrP. By contrast, more than 50% of the synthesized PrP was unglycosylated when the less efficient angiotensinogen (Ang) signal sequence (Kim et al, 2002) was used. The unglycosylated species of PrP for each construct was observed to be largely degraded within the 1-h chase period, a process that was retarded by proteasome inhibitors (Figure 2A). During this same time frame, the glycosylated PrP was trafficked to post-ER compartments, as evidenced by its decreased mobility due to Golgi-specific carbohydrate modifications. Thus, in the absence of proteasome inhibition, the pattern of PrP isoforms within 1 h after a pulse labeling is very similar between the wild-type, Opn-PrP, and Prl-PrP constructs (Figure 2A, top panel), consistent with their identical distributions at steady state. With proteasome inhibition, however, clear differences are observed in the amount of nonglycosylated PrP generated by differences in signal sequence efficiencies (Figure 2A, bottom panel). Figure 2.Reduced susceptibility to aggregate formation by modulation of PrP translocation. (A) Biosynthesis and maturation of PrP fused to different signal sequences was assessed by pulse-chase analysis (as described in Materials and methods) of transfected N2a cells in the absence (top panel) or presence (bottom panel) of proteasome inhibition. Samples after a 15 min pulse labeling ('P' lanes) and following a 1 h chase in unlabeled media ('C' lanes) are shown. The positions of different species of PrP are indicated: U, unglycosylated, *, singly glycosylated, **, doubly glycosylated, and M, mature. The percent of total PrP synthesized in the unglycosylated form is indicated below each set of lanes. (B) N2a cells expressing PrP with different signal sequences were assessed for aggregate formation by a solubility and sedimentation assay. The total proteins and immunoblots for GFP (a cotransfected control protein) and PrP in the soluble and insoluble fractions are shown (Sup. and Pel., respectively). The position of unglycosylated PrP (U) is indicated. Untreated and MG132-treated cells were analyzed in parallel. Cells not transfected with a PrP construct are indicated (nt). Download figure Download PowerPoint The consequences of this nontranslocated population were explored further by determining the effects of prolonged proteasome inhibition. A concentration of proteasome inhibitor that only partially impairs the proteasome system (Supplementary Figure S5) was used to mimic the reduced degradative capacity thought to develop during the pathogenesis of various neurodegenerative diseases (Bence et al, 2001; Ciechanover and Brundin, 2003) or even the normal aging process (Gray et al, 2003). With PrP, proteasome impairment with any of three different inhibitors results in the gradual accumulation of nonglycosylated PrP that is resistant to solubilization with mild detergents (Figure 2B and Supplementary Figures S6 and S7A). Remarkably, the generation of this insoluble nonglycosylated form was effectively abolished with Opn-PrP or Prl-PrP (Figure 2B and Supplementary Figure S7A), even upon prolonged inhibition of the proteasome (for up to 8 h; Figure 3A). Figure 3.Effect of prolonged proteasome inhibition on the metabolism of PrP and Opn-PrP. (A) N2a cells transfected with either wild-type PrP (upper panel) or Opn-PrP (lower panel) were treated for between 0 and 8 h with 5 μM MG132 before lysis and fractionation into detergent-soluble (Sup.) and insoluble (Pel.) fractions and analysis by immunoblotting. Note that, with wild-type PrP, an insoluble, unglycosylated species of PrP accumulates over 8 h (U); this is not observed with Opn-PrP, which only shows soluble, mature PrP (M). Results identical to those observed with Opn-PrP were also seen for Prl-PrP (unpublished results; see also Figure 2B). (B) Effect of signal sequence on 'propagation' of cytoplasmic PrP aggregates. Two parallel plates each of N2a cells transfected with either wild-type PrP or Opn-PrP were treated for 2 h with 5 μM MG132. One plate (left panel) was harvested immediately and analyzed for PrP detergent solubility and aggregation. The second set of dishes was rinsed to remove the MG132, and incubated an additional 21 h in normal media (right panel) before harvesting for analysis of PrP detergent solubility and aggregation. After a 2 h 'pulse' of MG132, note that the immunoblots of PrP and Opn-PrP are largely indistinguishable: both show nearly quantitative solubility and little unglycosylated, insoluble PrP. After a 21 h chase, wild-type PrP was nearly all unglycosylated and insoluble, as has been proposed to occur by a 'self-propagation' mechanism (Ma and Lindquist, 2002). Even under these conditions, however, relatively little Opn-PrP was found in the unglycosylated, insoluble form. Download figure Download PowerPoint Furthermore, small amounts of cytosolic PrP generated upon transient treatment with proteasome inhibitors have been reported to accumulate substantially over time by 'self-propagation' (Ma and Lindquist, 2002). Although the mechanism of 'propagation' is not yet clear, this treatment protocol allows the detection of very small amounts of cytosolic PrP, which presumably serves as a seed for subsequent accumulation. Under such propagation conditions that cause nearly all of the cellular PrP to become cytosolic, very little Opn-PrP accumulated in the unglycosylated form (Figure 3B). Thus, modulating PrP translocation by using a more efficient signal sequence can substantially reduce or even eliminate the ability of PrP to form nonglycosylated cytosolic aggregates under a variety of conditions. The consequences of modulating PrP translocation were also examined in live cells expressing wild type and/or Opn-PrP tagged with different color variants (either cyan or yellow) of monomerized green fluorescent protein (mGFP). Co-expression of these two constructs in cultured cells demonstrated them to have the same localization as both each other and nontagged PrP (Figure 4A and Supplementary Figure S2), with the majority of PrP being found on the cell surface and lesser amounts in an intracellular pool colocalizing with the Golgi and endosomal compartments (unpublished results; see also Figure 5A and C). Biochemical analyses confirmed that fluorescent protein-tagged PrP and Opn-PrP were identically glycosylated and resistant to deglycosylation with endoglycosidase H (unpublished results). Upon inhibition of the proteasome in cells expressing PrP-mYFP or Opn-PrP-mYFP, a striking difference was observed (Figure 4B). Numerous PrP-expressing cells began to show a different pattern of localization, with an increasing intracellular population (Figure 4B, left panels). Quantitative single-cell analyses (Figure 4C, closed circles) revealed that the ratio of surface to intracellular localized PrP was lowest for the cells expressing the most PrP. For the lowest-expressing cells, only a modest increase in the intracellular population was observed. Parallel analysis of Opn-PrP-expressing cells revealed them to maintain the normal pattern of subcellular localization, regardless of expression levels (Figure 4B, right panels). Indeed, only a very small difference in the intracellular to surface ratio was observed even in the highest expressing cells (Figure 4C, open circles). Similar results were obtained with indirect immunofluorescence analysis of cells expressing nontagged versions of PrP and Opn-PrP (unpublished results). Figure 4.Visualization and modulation of PrP metabolism in single live cells. (A) Expression and colocalization of PrP-mYFP (red) and Opn-PrP-mCFP (green) in N2a cells. Similar results were obtained with PrP-mCFP and Opn-PrP-mYFP (unpublished results). (B) Comparison of localization patterns in N2a cells expressing PrP-mYFP or Opn-PrP-mYFP after proteasome inhibition (with MG132) for 8 h. Two exposures (upper and lower panels) are shown for each representative field to facilitate visualization of cells expressing high (arrows) and low (asterisks) levels of PrP. The PrP-mYFP and Opn-PrP-mYFP cells were visualized using identical imaging conditions to allow direct comparisons between them. (C) Quantitative analysis of subcellular localization relative to expression level for PrP-mYFP (closed circles) and Opn-PrP-mYFP (open circles) after proteasome inhibition as in panel B. (D) Visualization of PrP-mYFP (red) and Opn-PrP-mCFP (green) following proteasome inhibition. Download figure Download PowerPoint Figure 5.Analysis of PrP and Opn-PrP localization. N2a cells were transfected with Opn-PrP-mYFP (panels A and B) or PrP-mYFP (panels C and D) and either left untreated (panels A and C) or treated with 5 μM MG132 for 4 h. The cells were fixed and processed for double immunofluorescence analysis using antibodies against an endosome marker (EEA1, in red) and a Golgi marker (beta-COP, in blue). Green indicates the position of YFP-tagged PrPs. Note that, in panels A–C, mYFP is localized primarily to the cell surface and perinuclear structures that overlap partially with Golgi and endosome structures. By contrast, panel D shows many cells (indicated with arrows) that have additional accumulations of PrP in other regions of the cytoplasm. Higher magnification images with separated color channels of representative cells from panels B and D are shown in Supplementary Figures S8 and S9, respectively. Download figure Download PowerPoint When examined in the same cell, a difference in PrP-mYFP and Opn-PrP-mCFP localization upon proteasome inhibition was readily apparent within 2–4 h (Figure 4D). Opn-PrP-mCFP, similar to before proteasome inhibition (compare to Figure 4A), continued to show localization at the cell surface and perinuclear structures. By contrast, PrP-mYFP now clearly showed additional accumulations in the cytoplasm that did not colocalize with Opn-PrP-mCFP (Figure 4D). At longer times after proteasome inhibition, this differential localization was more difficult to see, apparently due to substantial changes in cellular morphology caused by the accumulating PrP-mYFP (unpublished results). That notwithstanding, the ability to observe a difference in the localization for a population of PrP distinct from Opn-PrP within the same cell directly demonstrates that the trafficking pathways diverge for a discrete proportion of these two proteins. Hence, the differential consequences of the PrP and Opn signals on substrate translocation can be directly visualized in live cells during conditions of a compromised proteasome. Colocalization studies showed that, both before and after proteasome inhibitor treatment, the majority of Opn-PrP-mYFP was at the cell surface and in perinuclear structures consistent with Golgi and endosomes (Figure 5A and B and Supplementary Figure S8). By contrast, a significant proportion of the PrP-mYFP that accumulates after proteasome inhibition is found in cytoplasmic regions that do not colocalize with either Golgi or endosomes (Figure 5D and Supplementary Figure S9). Thus, PrP and Opn-PrP show essentially identical cellular localization patterns before (Figures 4A, 5A and C and Supplementary Figure S2), but not after proteasome inhibition (Figures 4D, 5B and D and Supplementary Figures S8 and S9). We conclude that the additional cytoplasmic accumulations of PrP-mYFP (but not Opn-PrP-mYFP) seen by microscopy are likely to correspond to the unglycosylated PrP that accumulates upon proteasome inhibition in biochemical experiments (e.g., Figures 2 and 3). Generation of cytosolic PrP independently of expression level The single-cell analysis of PrP localization (Figure 4C) revealed that, even at relatively low levels of expression, a difference between PrP and Opn-PrP could be observed. This suggests that the basis of cytosolic PrP generation is not likely to be a consequence of saturating the ER targeting and translocation machinery. This conclusion was confirmed in two ways. First, we demonstrated that gross overexpression of Prl-PrP ∼5–10 fold above wild-type PrP still did not generate the aggregated nonglycosylated form of PrP upon proteasome inhibition (Figure 6A and B). Thus, generation of unglycosylated PrP cannot be induced simply as a consequence of overexpression if a sufficiently efficient signal sequence is used for translocation. This indicates that even very high amounts of PrP in the ER lumen are insufficient to result in the generation of unglycosylated PrP by retrotranslocation. Figure 6.Effect of expression level on cytosolic PrP generation. (A) N2a cells were transfected with either wild-type PrP or a larger amount of Prl-PrP. In both cases, equal amounts of a plasmid expressing GFP were cotransfected. A fixed amount of cell lysate from PrP-expressing cells (1 ×; lane 2) was compared to different amounts of cell lysate from the Prl-PrP-expressing cells (from 0.1 × to 1 ×; lanes 3–6). Cells transfected with just GFP (nt; lane 1) are also shown as a control. Immunoblots were probed with antibodies against PrP, GFP, and an endogenous protein, the beta subunit of Sec61. Note that GFP and Sec61 levels are comparable when equal amounts of nt, PrP, and Prl-PrP cell lysates are compared (lanes 1–3). By contrast, Prl-PrP is expressed at ∼5–10-fold higher levels than PrP, as indicated by the observation that equal intensities are observed when 0.1–0.2 × of Prl-PrP (lanes 5 and 6) is compared to 1 × of PrP (lane 2). (B) Duplicate dishes of cells from lanes 1 and 2 of panel A were either left untreated or treated for 4 h with 5 μM MG132 prior to harvesting and fractionation of detergent-soluble PrP (Sup.) from insoluble PrP (Pel.). One-tenth the amount of Prl-PrP lysate was analyzed on the gel to allow direct comparisons between the samples. Note that, with PrP, unglycosylated, insoluble PrP (U) is generated upon MG132 treatment. However, despite the ∼10 × overexpression of Prl-PrP, this form is not generated. (C) Effect of proteasome inhibition on endogenously expressed PrP. Untransfected N2a cells were treated for between 0 and 8 h with 5 μM MG132 before analysis of total proteins by immunoblotting with the 7D9 anti-PrP antibody (which detects mouse PrP). Note that an unglycosylated species of PrP (U) accumulates over 8 h (left panel). Samples from the 8 h treatment were also analyzed for PrP detergent solubility and aggregation (right panel). Note that a substantial fraction of the unglycosylated PrP is in the detergent insoluble pellet (P), while all of the mature, glycosylated PrP (M) is in the detergent-soluble supernatant (S). Download figure Download PowerPoint In a second approach, we were able to observe the accumulation of nonglycosylated aggregates of the endogenously expressed PrP in nontransfected N2a cells treated with proteasome inhibitors (Figure 6C). Thus, the progressive generation and accumulation of insoluble, unglycosylated PrP upon proteasome inhibition is not a consequence of exogenous overexpression or the use of a viral promoter, as has been suggested previously (Drisaldi et al, 2003). Many of the conclusions from these previous studies rested on the assumption that cytoplasmic, unglycosylated PrP would necessarily contain an uncleaved signal sequence if it were generated by aborted translocation. However, our previous studies indicate that the step at which some signals are inefficient in initiating translocation involves a post-targeting interaction with the Sec61-translocon (Kim et al, 2002). As the signal peptidase complex is at the translocon and removes signals cotranslationally (Rapoport et al, 1996), the absence of the signal peptide may not necessarily be a reliable indicator of a protein having been completely translocated into the ER lumen. Examples of cytosolic localization after signal sequence cleavage of aborted translocation intermediates have been described previously (Garcia et al, 1988; Ou et al, 1989; Ooi and Weiss, 1992). Indeed, we have found that, in vitro, cytoplasmic PrP containing a cleaved signal can be generated by chains that were targeted to, but only partially translocated through the translocon before completing synthesis in the cytosol (unpublished results). Consistent with this observation, we often see two unglycosylated species of PrP, separated by approximately the size of the PrP signal peptide, that accumulate to varying degrees in proteasome inhibited cells (e.g., see Supplementary Figure S6). Thus, the use of an anti-signal peptide antibody (Drisaldi et al, 2003; Stewart and Harris, 2003) as a sole marker for cytoplasmic PrP is unreliable, calling into question the interpretation of negative results that failed to detect signal-containing PrP in primary neurons or diseased brain (Stewart and Harris, 2003). Conversely, the conclusion (Ma and Lindq
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