Portal de Periódicos da CAPES

Expression Cloning of an Interferon-inducible 17-kDa Membrane Protein Implicated in the Control of Cell Growth

1995; Elsevier BV; Volume: 270; Issue: 40 Linguagem: Inglês

10.1074/jbc.270.40.23860

1083-351X

Gisèle A. Deblandre, Olivier Marinx, Sharon S. Evans, Samira Majjaj, Oberdan Léo, Daniel Caput, Georges Huez, Marc Wathelet,

Neutrophil, Myeloperoxidase and Oxidative Mechanisms

Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity of interferons based on (1Gutterman J.U. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 1198-1205Crossref PubMed Scopus (678) Google Scholar) their pattern of induction in interferon-sensitive and -resistant cell lines and (2De Maeyer E. De Maeyer-Guignard J. Interferons and Other Regulatory Cytokines. John Wiley & Sons, New York1988: 91-113Google Scholar) the ability of a membrane fraction enriched in 17-kDa proteins to inhibit cell growth. To gain insight into the nature of the proteins that mediate the antiproliferative activity of interferons, a monoclonal antibody, 13A5, was generated that reacted specifically with a 17-kDa interferon-inducible cell surface protein. The expression pattern of this 17-kDa protein by human cell lines correlated with sensitivity to the antiproliferative activity of interferons. To obtain information regarding the structure of this protein, the 13A5 antibody was used to screen COS cells transfected with a human cDNA expression library. Sequence analysis of a full-length cDNA clone revealed identity with the 9-27 cDNA, previously isolated on the basis of its interferon inducibility by differential screening. In addition, the 17-kDa protein encoded by the 9-27 gene was shown to be identical to the Leu-13 antigen. Leu-13 was previously identified as a 16-kDa interferon-inducible protein in leukocytes and endothelial cells and is a component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals. These results suggest a novel level of cellular regulation by interferons involving a membrane protein, encoded by the interferon-inducible 9-27 gene, which associates with other proteins at the cell surface, forming a complex relaying growth inhibitory and aggregation signals. Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity of interferons based on (1Gutterman J.U. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 1198-1205Crossref PubMed Scopus (678) Google Scholar) their pattern of induction in interferon-sensitive and -resistant cell lines and (2De Maeyer E. De Maeyer-Guignard J. Interferons and Other Regulatory Cytokines. John Wiley & Sons, New York1988: 91-113Google Scholar) the ability of a membrane fraction enriched in 17-kDa proteins to inhibit cell growth. To gain insight into the nature of the proteins that mediate the antiproliferative activity of interferons, a monoclonal antibody, 13A5, was generated that reacted specifically with a 17-kDa interferon-inducible cell surface protein. The expression pattern of this 17-kDa protein by human cell lines correlated with sensitivity to the antiproliferative activity of interferons. To obtain information regarding the structure of this protein, the 13A5 antibody was used to screen COS cells transfected with a human cDNA expression library. Sequence analysis of a full-length cDNA clone revealed identity with the 9-27 cDNA, previously isolated on the basis of its interferon inducibility by differential screening. In addition, the 17-kDa protein encoded by the 9-27 gene was shown to be identical to the Leu-13 antigen. Leu-13 was previously identified as a 16-kDa interferon-inducible protein in leukocytes and endothelial cells and is a component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals. These results suggest a novel level of cellular regulation by interferons involving a membrane protein, encoded by the interferon-inducible 9-27 gene, which associates with other proteins at the cell surface, forming a complex relaying growth inhibitory and aggregation signals.